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PathHunter™ JAK3 U2O Activity Cell Line

PathHunter JAK3 U2P Activity Cell Lines are used with the PathHunter Detection Kit (93-0001) for measuring the activation of the target G Protein-Coupled Receptor (GPCR). Each cell line is engineered to co-express the ProLink/Enzyme Donor (PK)-tagged GPCR and the Enzyme Activator (EA)-tagged β-Arrestin fusion proteins. Upon GPCR activation, the two enzyme fragments are brought together, forming an active -Gal enzyme through Enzyme Fragment Complementation (EFC).

Catalog No. Size Price Qty    
93-0796C3 50ml LOGIN TO VIEW
1
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How it works
  • Target/Description
  • Group
  • SH2 Domain
  • Cell Line
  • CRBD
  • Species
  • Accession Number
  • Vector Number
  • Available as a Service
  • Janus kinase 3
  • TK
  • PLCG2
  • CHO-K1
  • JAK2 Amino Acid Range: (aa 1 - 255)
  • Human
  • BC039695
  • PK1
  • Yes

Figure 1. PathHunter® Cells were plated in a 384-well plate at 5,000 cells/well and stimulated with the inhibitor (referenced above) for the time and temperature specified below. Agonist was added to the plates and incubated at conditions specified below. Signal was detected using the PathHunter® Detection Kit (93-0001) according to the recommended protocol. Please refer to the User manual for more details.

Assay Information

  • Control Agonist
  • Specifications
  • Control Agonist
  • CP-690550
  • IC50 of the Inhibitor (nM): 792.6
  • Prolactin

Specifications

Innovative Solutions for Research in Electrophysiology

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Product References

Innovative Solutions for Research in Electrophysiology

Cortech Solutions is the single resource for all the tools a neuroscientist needs for advanced brain research. We identify best-in-class solutions, work with the manufacturers to ensure compatibility, and offer the total solution with a single source for technical support.

Leave the technical solutions to us...

You Concentrate on the science!

 

Manuals & Protocols

Innovative Solutions for Research in Electrophysiology

Cortech Solutions is the single resource for all the tools a neuroscientist needs for advanced brain research. We identify best-in-class solutions, work with the manufacturers to ensure compatibility, and offer the total solution with a single source for technical support.

Leave the technical solutions to us...

You Concentrate on the science!

 


Product Name Catalog No. Size Price Qty  
PathHunter® select U2OS Cell Culture Kit-L 92-0018GL3 50ml $50.00
1
Add to Cart
PathHunter® Cell Planting 16 Reagent 93-0563R16A 250ml $275.00
1
Add to Cart
PathHunter® Detection Kit 93-0001 50ml $50.00
1
Add to Cart