Signaling Pathways

Interrogate Target-Specific Pathways with a Suite of Cellular Assays

Eurofins DiscoverX offers a comprehensive collection of cell-based pathway indicator assays designed to detect activation or inhibition of complex signal transduction pathways in response to a small molecule or biologics. Based on the established PathHunter® technology, these cell lines allow you to measure distinct events within a variety of pathways involved in immune response, compound toxicity, cholesterol metabolism, antioxidant function, DNA damage, ER stress, and more. In combination with cell lines and fast and simple chemiluminescent detection, these signaling pathway assays will help you generate cellular pathway selectivity profiles and gain a full understanding of your compounds or biologics for receptor-proximal or -distal events without relying on complex phenotypic screens

Learn more about the updated Cell Culture and Handling Procedure in the new Technical Bulletin.


Cell Lines

Explore our growing panel of stable, target-specific, pathway indicator cell lines for assays to measure activation or inhibition of novel signaling pathways in response to compound treatment.
How pathway assays work >

Assay-Ready Kits

PathHunter® eXpress Pathway assays are complete, ready-to-use kits, including all cells and reagents to measure pathway activation or inhibition in live cells. Free yourself from lengthy cell culture and expensive instrumentation!
Learn about Assay Ready eXpress Kits >

Cell Culture Kits, Reagents & Consumables

Performance you can trust! Get the highest quality data from your cell-based pathway assays in combination with our lab-certified PathHunter® cell culture media, tissue culture reagents, and plates.

Ligands & Inhibitors

Designed to target a specific signaling pathway, our purified, reference ligands and inhibitors are a convenient and cost-effective way to validate any stable pathway cell line or assay-ready kit.
View SMKI reference compound data >
Learn about competition binding assays >

Detection Reagents

Get optimal assay performance using certified chemiluminescent or fluorescent PathHunter® Detection Reagents that have been validated for use in both 96-well and 384-well microplate formats.