Nuclear Protein Product Solutions

Cell-based Assays to Evaluate Activation and Translocation of Nuclear Proteins

Nuclear proteins are key targets for drug development due to their ubiquitous roles in controlling cellular behavior and pathway expression. The translocation of many nuclear proteins into and out of the nucleus is a highly controlled mechanism that can dictate protein function. Controlling this translocation behavior using therapeutics, or ensuring that a potential drug does not cause undesired effects on translocation, are important applications for measuring nuclear translocation.

Nuclear hormone receptors (NHRs), including steroid receptors, are a critical subset of nuclear proteins that regulate gene expression in response to ligand activation and are intimately involved in endocrine pathway signaling. NHRs commonly rely on a coactivator or corepressor protein, often a steroid receptor cofactor (SRC protein), to perform their function. Enhancing or disrupting the association of these proteins can be a target for therapeutic development focused on controlling NHR-related gene expression.

Eurofins DiscoverX^® provides PathHunter^® nuclear translocation and protein interaction cell-based, functional assays to investigate protein translocation events and activation of nuclear proteins. Nuclear translocation assays allow for studying the trafficking of your protein of interest to and from the nucleus and analyzing the pharmacological effects of therapeutics on these events. Protein interaction assays can be used to evaluate the interaction of NHRs and their cofactors, including therapeutics that affect this protein-protein interaction.

Among the advantages of the Eurofins DiscoverX platform is that, unlike other assays, these cell-based assays do not require antibodies, fluorescent tags, or specialized equipment, and they provide an easy-to-use, high-throughput capable, and quantitative measurement of protein-protein interaction.