Custom Assay Development Services
Assay development solutions tailored to your needs
When existing off-the-shelf solutions are not enough, trust DiscoverX to develop a solution that will produce the quality results you expect. DiscoverX offers a range of custom development services including bioassays for drug discovery, process development and basic research. Our team of molecular and cellular biologists will work with you to match one of our proprietary platforms such as PathHunter®, KINOMEscan®, InCELL Hunter™, InCELL Pulse™, or HitHunter® to deliver a cellular or biochemical solution that provides the quality results you expect.
Available Services
- Orthologs and mutants
- Custom cell backgrounds
- Custom cell lines
- Protein overexpression
- Kit formulation/packaging
|
|
|
For more information about our services, select one of the tabs below.
PathHunter® Cell-based Assay Development Services
|
|
PathHunter is a versatile cell-based assay platform, based on our proprietary EFC technology, successfully employed to develop validated assay solutions for over 300 targets spanning many target classes including GPCRs, Kinase, NHRs, and more. Innovative application of this core technology, has enabled the development of robust, reliable, and high-throughput-friendly chemiluminescent assays to measure protein-protein interaction, protein translocation and degradation.
PathHunter CAD Services will work closely with you to develop a custom cell-based assay specific for your target, providing comprehensive solutions where you need them most. |
PathHunter - cell-based technology format can query many types of interactions
-
Measure inducible and constitutive protein interactions
-
Measure protein translocation to various intracellular locations in a
non-imaging HTS friendly assay
-
Accurately measure protein levels and stabilization
|
How it Works |
HitHunter® Biochemical Assay Development Services
|
|
The HitHunter Assay is a validated platform for biochemical quantification of second messenger signaling for cAMP and cGMP. Extension of the HitHunter principle to novel targets allows for investigation of protease and substrate activity. HitHunter assays are formatted as competitive immunoassays, where analytes are detected in solution, without separation or wash steps, providing improved sensitivity and precision compared to conventional immunoassays.
HitHunter Biochemical Assay Development Services will work closely with you to develop a custom assay to detect protease and substrate activity or measure an analyte, providing comprehensive solutions where you need them most. |
HitHunter biochemical technology format has these advantage
-
Develop novel assays for identifying analytes
-
Develop cleavage assays to assay protease activity
-
Develop assays for use with primary cells, etc
-
All assays will be HTS compatible, etc.
-
Chemiluminescent detection - minimizes false positives from auto fluorescence
-
Reliable and robust assay with high Z' (0.75)
-
HTS-friendly - adaptable to 1536 applications
-
Large assay windows
-
Broad application to multiple target classes
|
How it Works |
InCELL Assay Development Services
| |
InCELL Pulse and InCELL Hunter are novel, yet powerful in vitro cellular ligand binding assay platforms that can be used to determine compound binding in a native cellular environment. Both platforms use enzyme fragment complementation technology to measure binding to the protein of interest and are powerful development tools to evaluate target engagement for intractable targets where other technologies have been unsuccessful. |
InCELL – A simple cell-based assay for complex targets
- Cell-based assay broadly applicable to several target classes
- Target-specific response
- Direct measure of intracellular binding to target
- Activity independent
- HTS compatible
- Bridge between in vitro binding assay and cellular functional assay
|
How it Works |