Recombinant Kinase & Phosphatase Enzymes

Largest Portfolio of Purified Recombinant Proteins for Screening Inhibitors

Kinases are cell signaling enzymes that catalyze the transfer of a phosphate group from ATP to its substrate, while phosphatases are regulatory enzymes that remove the phosphate group. Both enzyme families play roles in the regulation of intracellular signaling pathways. There are ~535 kinases in the human kinome (eLife Sciences, 2021), and they are generally grouped into either serine/threonine, tyrosine, or lipid kinases. Kinase substrates include a variety of intracellular proteins and molecules, including other kinases, transcription factors, lipids, and other proteins. The phosphorylation and dephosphorylation of the substrate modifies its enzymatic activity. These activities include diverse cellular processes, including cell division, protein synthesis, transcriptional regulation, and neurotransmission. Activating mutations of kinases, or their dysregulation by upstream factors, that lead to either gain-of-function or loss-of-function are implicated in many diseases, including cancer.


Eurofins DiscoverX provides a new home for the original Millipore (Upstate) kinase enzyme portfolio covering over 70% of the kinome. View the comprehensive portfolio of over 420 active, mutant, and inactive recombinant kinases available in multiple pack sizes, including bulk sizing, to provide you with scalable options. Dissect the physiological role of kinases and phosphatases, screen for inhibitors, or perform enzymatic kinases or phosphatase activity assays. Access the same enzymes used daily in the renowned KinaseProfiler™ and PhosphataseProfiler™ services, and receive the quality and confidence that comes with screening 100s of millions of data point generated over the past several decades.

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Advantages of Recombinant Proteins

  • Comprehensive Portfolio - Over 420 high quality active, inactive, unactive, and mutant kinases, including many exclusive enzymes including the PI3K and the PIKK group (ATM, ATR, mTOR, and DNA-PK)
  • Optimal Expression Systems - Enzymes produced in their optimal system (insect, mammalian, or bacterial) to give the correct characteristics for activity and inhibition
  • High Quality - Rigorous quality control procedure ensures the highest purity (>95%), specific activity, and lot-to-lot consistency
  • Multiple Sizes - Multiple pack sizes and bulk sizing available to provide you with flexible options from a one-time experiment to a full-blown HTS campaign

Key Resources


  • Kinases – View over 420 active, inactive, and unactivated kinases available as human, mouse, rat, and other species; expressed from bacterial, insect, or mammalian cells
  • Phosphatases – Utilize the same phosphatase, expressed in E. coli and tagged (e.g. GST, His6) or untagged, in your discovery that are used in the renowned PhosphataseProfiler drug discovery services

Custom and Toolbox Products

  • Custom Capabilities – Custom cell lines, assays, and protein development capabilities optimized to your requirements.

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