Find a Product

Receptor Dimerization Assays

Quantitative assays for receptor dimer formation

Ligand-induced receptor dimerization is the first step following receptor activation and the most proximal and reliable indicator of receptor activation. Until now, these interactions have not been successfully monitored using classic cellular protein interaction techniques. The PathHunter® Dimerization assay platform enables you to measure these interactions on a cell surface and is ideal for lead identification, quality control and development of neutralizing antibody (Nab) assays for a variety of biologics, including bi-specific antibodies. View Validation Data »
 
  View List of Targets
See How it Works

Assay Benefits & Highlights

  • Functional target specific response
  • Simple one-step, plate-treat-read protocol
  • Validated for the development of biologics
  • Flexible - use for screening, potency and Nab assays

Dimerization Assay Principle

The two target receptors are tagged with ProLink™ (PK) or Enzyme Acceptor (EA). Upon ligand-induced activation, the receptors dimerize forcing the two β-gal components to complement and create an active enzyme. Active β-gal generates a chemiluminescent signal in the presence of substrate.

Screen & optimize peptides, antibodies or even small molecules

Profile & optimize bi-specific antibodies Create cell-based assays for heterodimer pairs based on native receptor biology