Functional Screening and Cellular Potency Assays

Identify functional biologics in bacterial lysates and hybridoma supernatants

PathHunter® assays are designed to accurately identify functional biologics from bacterial lysates and hybridoma supernatants in a robust and highly sensitive manner. These assays maintain native receptor biology and provide an accurate, quantitative and reproducible target-specific readout, resulting in a best-in-class lead identification and lead selection tool for biologics.

 

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High Tolerance of Bacterial Lysates and Hybridomas
 

GIPR Assay in Bacterial Lysate

   

HRH1 Assay in Human Serum

   

Anti-CCL20 in Serum

   
High tolerance of complex biological samples PathHunter assays can detect functional biologics in various samples, such as human serum, plasma, hybridoma lysates and bacterial lysates. Above, we validated with GIPR assay with up to 8.5% bacterial lysate (left panel), the HRH1 assay with up to 10% normal human serum (middle panel) and demonstrate robust detection of antibody-based inhibition of the CCL20 ligand in the presence of 80% serum without loss of sensitivity (right panel).

See how the assay works

View slides explaining how these assays work to conduct functional screening and determine cellular potency.


View Validation Data  

Types of Biologics Developed with PathHunter Assays
 

Receptor Agonists

   

Anti-Receptor Antibodies

   

Anti-Ligand Antibodies

   
These assays can be used to identify, screen and profile both small and large molecules.  Identify agonists (Left panel) for any receptor such as the Growth Hormone Receptor.  Identify novel anti-receptor antibodies (Middle panel), e.g., a series of commercially available anti-receptor, blocking antibodies show a dose-dependent inhibition of ErbB1 activity in U2OS cells.  Identify novel anti-ligand antibodies (Right panel), e.g., an anti-CCL20 antibody shows a dose-dependent inhibition of human CCR6.