Workflow protocol schematic to show how to generate an EFC cell-based assay. Start with an engineered PathHunter® parental cell line containing EA to study subcellular translocation (e.g. including membrane receptor trafficking), SH2 recruitment for receptor kinases, or GPCR β-arrestin recruitment. The specific parental cell line chosen determines the assay type. Then create an ED-tagged target protein plasmid followed by transfection of this plasmid into the parental cell line containing EA. Perform antibiotic selection, detect the level of expression of the two fusion proteins (optional), and perform the desired assay in the presence of a ligand.
Custom Proteins, Cell Lines, and Cell-Based Assays Developed For You
When existing off-the-shelf solutions are not enough or a developer is not available, trust Eurofins DiscoverX to develop a solution that will product the quality results you expect. These programs include custom protein production through cell line and assay development. Cell lines and cell-based assays include unique backgrounds, orthologs, and mutants as well as creation of custom cellular (functional or binding) or biochemical assays for drug discovery, process development, and basic research.
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Webinars
Create Your Own Cell-Based Assays to Study Receptor Tyrosine Kinase Function
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Creating Cellular Target Engagement Assays for Diverse Kinases Using InCELL Pulse™
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Create Your Own InCELL Pulse™ Cellular Compound-Target Engagement Assay
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Blog Articles
Do-It-Yourself: Benefits and Challenges of Creating Your Own Cell-Based Assays
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Ideal Methods to Study Cellular Transport of Membrane Proteins
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Why Study GPCR Arrestin Recruitment?
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Critical Needs in Cellular Target Engagement
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Resources
GPCR Products List
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PathHunter Build Your Own β-Arrestin Assays
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PathHunter Protein Translocation Assays: Track Cellular Movement of Proteins to Multiple Membrane Compartments
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KILR Cytotoxicity Assays: Specifically Measure Target Cell Death in Co-Cultures
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InCELL Target Engagement Assays: Universal Cell-Based Compound-Target Binding Assays
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InCELL Pulse™ Target Engagement Kinase Reference Set
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