Build Your Own Assay Tools
Complete Set of Tools to Build Your Own Stable Cell lines and Cell-Based Assays
Take advantage of DiscoverX’s robust proprietary Enzyme Fragment Complementation (EFC) technology to develop your own stable cell lines and cell-based assays to study protein-protein interactions, compound-target engagement, cytotoxicity, protein translocation, and much more. Design your own workflows and utilize engineered parental cell lines, retroparticles, and plasmid vectors to introduce your own target of interest and create cell-based assays for multiple applications.
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Create Your Own Cellular Target Engagement Assay
Watch this 45-minute webinar to learn how to create your own cell-based assays to investigate compound-target engagement. Use these assays to validate hits identified in biochemical assays, measure cellular EC50 values, and rank compounds – all in the native cellular environment with no need for custom chemical tracer or antibody reagents.
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Rapidly Create β-Arrestin, Tyrosine Kinase, and Translocation Assays Using Engineered Cells
PathHunter® Parental Cell Lines
Utilize engineered PathHunter parental cells to rapidly develop β-arrestin recruitment, SH2-protein recruitment, or translocation EFC cell-based assays after introducing your protein of interest. These parental cell lines provide the ability to study β-arrestin recruitment with your favorite GPCR, analyze tyrosine kinase activity and SH2-recruitment, or investigate trafficking of your target protein to the plasma membrane, nucleus, or endosome.
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Easily Make Your Own Compound-Target Engagement Cell-Based Assays
InCELL Pulse™ Target Engagement Starter Kit
Develop cellular InCELL Pulse compound-target engagement assays to measure inhibitor cellular potency against user-defined protein targets of interest. These assays apply EFC and ligand-dependent thermal stabilization of target proteins such as protein kinases, methyltransferases, and hydrolases. Employ the simple protocol with minimal hands-on time to obtain quantitative inhibitor EC50 values in a the native cellular environment.
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Create Target-Specific, Stable β-Arrestin Cell-Based Assays
PathHunter β-Arrestin Retroparticles
Make your own stable β-arrestin cell lines and EFC cell-based assays for any target in any dividing cell type. Use the assays to analyze the activation and desensitization of GPCRs and regulation of other signaling molecules such as protein kinases that involve β-arrestin recruitment.
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Design Target Cells Expressing Receptor Antigens of Choice to Study Cytotoxicity
KILR® Retroparticles
Generate target cells, expressing natively expressed or engineered surface antigens of choice, in the cells of your choice, to measure target cell death in co-cultures. These designer cytotoxicity assays allow for studying antibody dependent cell-mediated cytotoxicity, complement dependent cytotoxicity, antibody dependent cell phagocytosis, and more.
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Clone Your Protein of Interest into EFC-Based Cloning Vectors
PathHunter ProLink™ and ProLabel® Cloning Vectors
Utilize pCMV Mammalian cloning vectors to clone in your protein of interest with ProLink or ProLabel, as an N- or C-terminal tag, and subsequently transfecting into a cell line to create EFC cell-based assays.
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EFC Vectors Expressing Targets Found in DiscoverX Cell Lines
Expression Vectors
Introduce an expression vector into unique cell types to create EFC cell-based assays. These vectors include a protein target found in our DiscoverX cell-based assays tagged with a ProLink or ProLabel sequence.
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Custom Assay Development Services
Don't see your solution? We can develop custom stable cell lines or cell-based assays for you.
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Webinars
Creating Cellular Target Engagement Assays for Diverse Kinases Using InCELL Pulse™
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Create Your Own InCELL Pulse™ Cellular Compound-Target Engagement Assay
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Make Your Own Cell-Based Assays to Study β-Arrestin Recruitment
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Blog Articles
Ideal Methods to Study Cellular Transport of Membrane Proteins
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Why Study GPCR Arrestin Recruitment?
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Critical Needs in Cellular Target Engagement
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Resources
PathHunter Build Your Own β-Arrestin Assays
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PathHunter Protein Translocation Assays: Track Cellular Movement of Proteins to Multiple Membrane Compartments
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KILR Cytotoxicity Assays: Specifically Measure Target Cell Death in Co-Cultures
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InCELL Target Engagement Assays: Universal Cell-Based Compound-Target Binding Assays
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InCELL Pulse™ Target Engagement Kinase Reference Set
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