Applications & Products / Build Your Own Assay Tools

Build Your Own Assay Tools

Complete Set of Tools to Build Your Own Stable Cell lines and Cell-Based Assays

Take advantage of DiscoverX’s robust proprietary Enzyme Fragment Complementation (EFC) technology to develop your own stable cell lines and cell-based assays to study protein-protein interactions, compound-target engagement, cytotoxicity, protein translocation, and much more. Design your own workflows and utilize engineered parental cell lines, retroparticles, and plasmid vectors to introduce your own target of interest and create cell-based assays for multiple applications.

 

Create Your Own RTK Functional, Cell-Based Assays

Watch this 30-minute webinar to learn how to make your own receptor tyrosine kinase (RTK) cell-based assays to analyze RTK function, dimerization, and translocation as well as screening of RTK therapeutics. Cytokine receptors and cytosolic (CTK) cell-based assays are discussed.

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Rapidly Create β-Arrestin, Tyrosine Kinase, and Translocation Assays Using Engineered Cells

PathHunter® Parental Cell Lines

Utilize engineered PathHunter parental cells to rapidly develop β-arrestin recruitment, SH2-protein recruitment, or translocation EFC cell-based assays after introducing your protein of interest. These parental cell lines provide the ability to study β-arrestin recruitment with your favorite GPCR, analyze tyrosine kinase activity and SH2-recruitment, or investigate trafficking of your target protein to the plasma membrane, nucleus, or endosome.

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Easily Make Your Own Compound-Target Engagement Cell-Based Assays

InCELL Pulse™ Target Engagement Starter Kit

Develop cellular InCELL Pulse compound-target engagement assays to measure inhibitor cellular potency against user-defined protein targets of interest. These assays apply EFC and ligand-dependent thermal stabilization of target proteins such as protein kinases, methyltransferases, and hydrolases. Employ the simple protocol with minimal hands-on time to obtain quantitative inhibitor EC50 values in a the native cellular environment.

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Create Target-Specific, Stable β-Arrestin Cell-Based Assays

 

PathHunter β-Arrestin Retroparticles

Make your own stable β-arrestin cell lines and EFC cell-based assays for any target in any dividing cell type. Use the assays to analyze the activation and desensitization of GPCRs and regulation of other signaling molecules such as protein kinases that involve β-arrestin recruitment.

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Design Target Cells Expressing Receptor Antigens of Choice to Study Cytotoxicity

KILR® Retroparticles

Generate target cells, expressing natively expressed or engineered surface antigens of choice, in the cells of your choice, to measure target cell death in co-cultures. These designer cytotoxicity assays allow for studying antibody dependent cell-mediated cytotoxicity, complement dependent cytotoxicity, antibody dependent cell phagocytosis, and more.

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Clone Your Protein of Interest into EFC-Based Cloning Vectors

PathHunter ProLink™ and ProLabel® Cloning Vectors

Utilize pCMV Mammalian cloning vectors to clone in your protein of interest with ProLink or ProLabel, as an N- or C-terminal tag, and subsequently transfecting into a cell line to create EFC cell-based assays.

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EFC Vectors Expressing Targets Found in DiscoverX Cell Lines

Expression Vectors

Introduce an expression vector into unique cell types to create EFC cell-based assays. These vectors include a protein target found in our DiscoverX cell-based assays tagged with a ProLink or ProLabel sequence.


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Custom Assay Development Services

Don't see your solution? We can develop custom stable cell lines or cell-based assays for you. Learn More
 

Webinars

Create Your Own Cell-Based Assays to Study Receptor Tyrosine Kinase Function  Watch Webinar
Creating Cellular Target Engagement Assays for Diverse Kinases Using InCELL Pulse™ Watch Webinar
Create Your Own InCELL Pulse™ Cellular Compound-Target Engagement Assay Watch Webinar
Make Your Own Cell-Based Assays to Study β-Arrestin Recruitment Watch Webinar
 

Blog Articles

Do-It-Yourself: Benefits and Challenges of Creating Your Own Cell-Based Assays Read Article
Ideal Methods to Study Cellular Transport of Membrane Proteins Read Article
Why Study GPCR Arrestin Recruitment? Read Article
Critical Needs in Cellular Target Engagement Read Article
 

Resources

GPCR Products List  PDF Download
PathHunter Build Your Own β-Arrestin Assays Download Flyer
PathHunter Protein Translocation Assays: Track Cellular Movement of Proteins to Multiple Membrane Compartments Download Flyer
KILR Cytotoxicity Assays: Specifically Measure Target Cell Death in Co-Cultures Download Flyer
InCELL Target Engagement Assays: Universal Cell-Based Compound-Target Binding Assays Download Flyer
InCELL Pulse™ Target Engagement Kinase Reference Set Download Booklet