Ready-to-Assay PRP/GPR10 Prolactin-Releasing Peptide Receptor Frozen Cells

Background Information:

The Ready-to-Assay PRP/GPR10 Prolactin-Releasing Peptide Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. prolactin releasing peptide) based activation of PRLHR GPCR activity via detection of increasing levels of intracellular calcium. The included cells overexpress PRLHR alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of PRLHR , the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format.

Target Class:

GPCR

Family:

Prolactin-Releasing Peptide

Sub Family:

Class A

Protein Name:

PRP

Protein Aliases:

PrRPR|G-protein coupled receptor 10|hGR3

Accession Number:

NM_004248

UniProt Number:

P49683

Gene Name:

PRLHR

Gene ID:

2834

Gene Aliases:

PrRPR

Target Species:

Human

Cell Background:

Chem-1