Ready-to-Assay NTR1 Neurotensin Receptor Frozen Cells

Background Information:

The Ready-to-Assay NTR1 Neurotensin Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. neurotensin) based activation of NTSR1 GPCR activity via detection of increasing levels of intracellular calcium. The included cells overexpress NTSR1 alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of NTSR1 , the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format.

Target Class:

GPCR

Family:

Neurotensin

Sub Family:

Class A

Protein Name:

NT1

Protein Aliases:

NTR1|High-affinity levocabastine-insensitive neurotensin receptor|NTRH

Accession Number:

NM_002531

UniProt Number:

P30989

Gene Name:

NTSR1

Gene ID:

4923

Gene Aliases:

NTR|NTRR

Target Species:

Human

Cell Background:

Chem-1