Ready-to-Assay Motilin Receptor Frozen Cells

Background Information:

The Ready-to-Assay Motilin Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. motilin) based activation of MLNR GPCR activity via detection of increasing levels of intracellular calcium. The included cells overexpress MLNR alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of MLNR , the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format.

Target Class:

GPCR

Family:

Motilin

Sub Family:

Class A

Protein Name:

Motilin

Accession Number:

NM_001507

UniProt Number:

O43193

Gene Name:

MLNR

Gene ID:

2862

Gene Aliases:

GPR38|MTLR|MTLR1

Target Species:

Human

Cell Background:

Chem-1