Ready-to-Assay GPR54/KiSS1 Receptor Frozen Cells

Background Information:

The Ready-to-Assay GPR54/KiSS1 Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand based activation of KISS1R GPCR activity via detection of increasing levels of intracellular calcium. The included cells overexpress KISS1R alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of KISS1R , the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format.

Target Class:

GPCR

Family:

Kisspeptin

Sub Family:

Class A

Protein Name:

KiSS1 (GPR54)

Protein Aliases:

G-protein coupled receptor 54|G-protein coupled receptor OT7T175|hOT7T175|Hypogonadotropin-1|Kisspeptins receptor|Metastin receptor

Accession Number:

NM_032551

UniProt Number:

Q969F8

Gene Name:

KISS1R

Gene ID:

84634

Gene Aliases:

HOT7T175|AXOR12|GPR54

Target Species:

Human

Cell Background:

Chem-1