Ready-to-Assay GnRH Gonadotropin-Releasing Receptor Frozen Cells

Background Information:

The Ready-to-Assay GnRH Gonadotropin-Releasing Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. gonadotropin-releasing hormone) based activation of GNRHR GPCR activity via detection of increasing levels of intracellular calcium. The included cells overexpress GNRHR alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of GNRHR , the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format.

Target Class:

GPCR

Family:

Gonadotrophin-Releasing Hormone

Sub Family:

Class A

Protein Name:

GnRH

Protein Aliases:

GnRH-R

Accession Number:

L03380

UniProt Number:

P30968

Gene Name:

GNRHR

Gene ID:

2798

Gene Aliases:

LHRHR

Target Species:

Human

Cell Background:

Chem-1