Ready-to-Assay FP Prostanoid Receptor Frozen Cells

Background Information:

The Ready-to-Assay FP Prostanoid Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. prostaglandin F2a) based activation of PTGFR GPCR activity via detection of increasing levels of intracellular calcium. The included cells overexpress PTGFR alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of PTGFR , the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format.

Target Class:

GPCR

Family:

Prostanoid

Sub Family:

Class A

Protein Name:

FP

Protein Aliases:

PGF2-alpha receptor|Prostanoid FP receptor

Accession Number:

NM_000959

UniProt Number:

P43088

Gene Name:

PTGFR

Gene ID:

5737

Gene Aliases:

FP

Target Species:

Human

Cell Background:

Chem-1