Ready-to-Assay D1 Dopamine Receptor Frozen Cells

Background Information:

The Ready-to-Assay D1 Dopamine Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. dopamine) based activation of DRD1 GPCR activity via detection of increasing levels of intracellular calcium. The included cells overexpress DRD1 alongside exogenous levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of DRD1 , the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format.

Target Class:

GPCR

Family:

Dopamine

Sub Family:

Class A

Protein Name:

D1

Protein Aliases:

Dopamine D1 receptor

Accession Number:

NM_000794

UniProt Number:

P21728

Gene Name:

DRD1

Gene ID:

1812

Target Species:

Human

Cell Background:

Chem-6