Ready-to-Assay CX3CR1 Chemokine Receptor Frozen Cells

Background Information:

The Ready-to-Assay CX3CR1 Chemokine Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. fractalkine) based activation of CX3CR1 GPCR activity via detection of increasing levels of intracellular calcium. The included cells overexpress CX3CR1 alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of CX3CR1 , the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format.

Target Class:

GPCR

Family:

Chemokine

Sub Family:

Class A

Protein Name:

CX3CR1

Protein Aliases:

CX3CR1|Beta chemokine receptor-like 1|CMK-BRL-1|CMK-BRL1|Fractalkine receptor|G-protein coupled receptor 13|V28

Accession Number:

U28934

UniProt Number:

P49238

Gene Name:

CX3CR1

Gene ID:

1524

Gene Aliases:

CMKDR1|V28|CCRL1|CMKBRL1|GPR13

Target Species:

Human

Cell Background:

Chem-4