Ready-to-Assay alpha2A Adrenergic Receptor Frozen Cells

Background Information:

The Ready-to-Assay α2A Adrenergic Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. epinephrine and norepinephrine) based activation of ADRA2A GPCR activity via detection of increasing levels of intracellular calcium. The included cells overexpress ADRA2A alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of ADRA2A , the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format.

Target Class:

GPCR

Family:

Adrenoceptor

Sub Family:

Class A

Protein Name:

alpha2A

Protein Aliases:

alpha-2AAR subtype C10| alpha-2A-adrenergic receptor

Accession Number:

NM_000681

UniProt Number:

P08913

Gene Name:

ADRA2A

Gene ID:

150

Gene Aliases:

ADRA2R|ADRAR

Target Species:

Human

Cell Background:

Chem-1