Ready-to-Assay alpha1B Adrenergic Receptor Frozen Cells

Background Information:

The Ready-to-Assay α1B Adrenergic Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. epinephrine and norepinephrine) based activation of ADRA1B GPCR activity via detection of increasing levels of intracellular calcium. The included cells overexpress ADRA1B alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of ADRA1B , the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format.

Target Class:

GPCR

Family:

Adrenoceptor

Sub Family:

Class A

Protein Name:

alpha1B

Protein Aliases:

Alpha-1B adrenoceptor

Accession Number:

NM_000679.3

UniProt Number:

P35368

Gene Name:

ADRA1B

Gene ID:

147

Target Species:

Human

Cell Background:

Chem-1