RBL-2H3 MRGPRX1 Gq Cell Line

Background Information:

The MRGPRX1 Gq cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. BAM (8-22)) based activation of MRGPRX1 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses MRGPRX1 , a naturally Gq coupled GPCR. Upon activation of MRGPRX1 , the resulting calcium activity can be fluorescently assayed using the Calcium NoWash PLUS Detection Kit.
This stable engineered cell line is provided as two vials of cryopreserved cells.

Target Class:

GPCR

Family:

Class A Orphan

Sub Family:

Orphan and other 7TM

Coupling:

Gq

Protein Name:

MRGX1 (MRGPRX1)

Accession Number:

NM_147199

UniProt Number:

Q96LB2

Gene Name:

MRGPRX1

Gene ID:

259249

Gene Aliases:

MRGX1|SNSR3|SNSR4

Target Species:

Human

Cell Background:

RBL-2H3