| Biological Information | |
|---|---|
| Background Information: | DiscoverX cell-based assays feature the powerful in vivo application of the established Enzyme Fragment Complementation (EFC) technology. In this approach, two complementing fragments; the small enzyme donor (ED) fragment and a larger enzyme acceptor (EA) fragment of the β-galactosidase (β-gal) enzyme are stably expressed as protein fusions in transfected cell lines. When ED binds to EA, complementation (EFC) occurs and β-gal activity is reconstituted and measured by substrate conversion to a luminescent product. The pCMV-ProLabel® and pCMV-ProLink™ mammalian cloning vectors are intended for mammalian cell expression of a GPCR or other protein of interest as a fusion protein with an ED [ProLabel (PL) or ProLink (PK)] tag. The resulting ED-tagged protein, upon interacting with an EA-tagged protein (most typically co-expressed in a EA-parental cell line), gives rise to EFC. Different ProLink and ProLabel tags have different affinities for the EA protein (and in the case of ARM-containing vectors, different affinities for Arrestin) and their selection and usage are described in detail in the pCMV ProLabel and pCMV-ProLink Mammalian Cloning Vectors User Manual. |
| Target Name: | N Vector |
| Usage | |
| Product Type: | Vectors |
| Application: | Drug Discovery & Development |
| Storage Conditions: | Store at –20°C until use. |
| Usage Disclaimer: | These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information. |
| Assay Information | |
| Detection Method: | Chemiluminescence |
| Additional Information | |
| Brand: | ProLabel® |
pCMV-ProLabel® N Vector
The pCMV-ProLabel® and pCMV-ProLink™ mammalian cloning vectors are intended for mammalian cell expression of a GPCR or other protein of interest as a fusion protein with an ED [ProLabel (PL) or ProLink (PK)] tag. The resulting ED-tagged protein, upon interacting with an EA-tagged protein (most typically co-expressed in a EA-parental cell line), gives rise to EFC.
User Manuals & Protocols
70-385 ProLabel and ProLink Mammalian Cloning Vectors REV1 User Manual
View DocumentDatasheets
93-0009 Datasheet
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