| Biological Information | |
|---|---|
| Background Information: | The PathHunter® TrkC Bioassay Kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay kit enables assessment of ligand (e.g. NT-3) based activation of TrkC receptors via detection of SH2 domain protein recruitment. The included cells overexpresses ProLink-tagged TrkC target protein and EA-tagged SH2 domain protein. Upon activation of the receptor by its ligand or an agonist antibody, recruitment of SH2 domain protein to the phosphorylated receptor tail leads to production of EFC signal. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates. |
| Target Class: | Kinase |
| Family: | Neurotrophic tyrosine kinase Receptor type 3 |
| Sub Family: | Protein Tyrosine |
| Accession Number: | NM_001012338 |
| Target Name: | TrkC |
| Target Aliases: | Neurotrophic tyrosine kinase receptor type 3, TRKC, GP145-TrkC, gp145(trkC) |
| Target Species: | Human |
| Cell Background: | U2OS |
| Usage | |
| Product Type: | Bioassay Kits |
| Application: | Potency; Commercial Release & Stability |
| Kit Components: |  |
| Storage Conditions: | Store in vapor phase of liquid nitrogen. |
| Usage Disclaimer: | These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information. |
| Assay Information | |
| Assay Type: | Functional |
| Assay Measures: | SH2 Recruitment |
| Detection Method: | Chemiluminescence |
| Additional Information | |
| Brand: | PathHunter® |
PathHunter® U2OS TrkC Bioassay Kit
The PathHunter® TrkC Bioassay Kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay kit enables assessment of ligand (e.g. NT-3) based activation of TrkC receptors via detection of SH2 domain protein recruitment.
User Manuals & Protocols
70-378 PathHunter TrkC Bioassay Kit REV0 User Manual
View DocumentDatasheets
93-0464Y3x Datasheet
View Document