PathHunter® U2OS TrkB-P75 Functional Assay

Background Information:

The PathHunter® U2OS TrkB-P75 Functional Assay provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting RTKs. This kit assesses RTK activation via detection of activated TrkB and P75 dimerization-induced SH2 domain protein recruitment. This product uses EFC technology. The included cell line overexpresses PK-tagged TrkB and an EA-tagged SH2 domain protein. It also overexpresses an untagged co-receptor, P75 Upon ligand-induced receptor activation, SH2-EA recruitment leads to production of EFC signal.
This stable engineered cell line is provided as two vials of cryopreserved cells.

Target Class:

Kinase

Family:

RTK

Sub Family:

Protein Tyrosine

Protein Name:

TRKB

Protein Aliases:

GP145-TrkB|Trk-B|Neurotrophic tyrosine kinase receptor type 2|TrkB tyrosine kinase|Tropomyosin-related kinase B

Accession Number:

NM_006180

UniProt Number:

Q16620

Gene Name:

NTRK2

Gene ID:

4915

Gene Aliases:

TRKB

Target Species:

Human

Cell Background:

U2OS