| Biological Information | |
|---|---|
| Background Information: | The PathHunter® TrkB-P75 Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses ligand (e.g. BDNF) induced dimerization of TrkB-P75 receptors. This product uses EFC technology. The included cells overexpress ProLink-tagged TrkB and an EA-tagged SH2 domain protein. It also overexpresses an untagged co-receptor, P75. Upon ligand-induced dimerization of TrkB-P75, detectable EFC signal is produced. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates. |
| Target Class: | Kinase |
| Family: | Neurotrophic tyrosine kinase Receptor type 2 |
| Sub Family: | Protein Tyrosine |
| Accession Number: | NM_006180 |
| Target Name: | TrkB-P75 |
| Target Aliases: | Neurotrophic tyrosine kinase receptor type 2OBHDTRKBDEE58trk-BEIEE58GP145-TrkB |
| Target Species: | Human |
| Cell Background: | U2OS |
| Usage | |
| Product Type: | Bioassay Kits |
| Application: | Potency, Commercial Release & Stability |
| Kit Components: |  |
| Storage Conditions: | Short term (24 h): Store in vapor phase of liquid nitrogen. |
| Usage Disclaimer: | These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information. |
| Assay Information | |
| Assay Type: | Functional |
| Assay Measures: | SH2 Recruitment |
| Detection Method: | Chemiluminescence |
| Additional Information | |
| Brand: | PathHunter® |
PathHunter® U2OS TrkB-P75 Bioassay Kit
The PathHunter® TrkB-P75 Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses ligand (e.g. BDNF) induced dimerization of TrkB-P75 receptors
User Manuals & Protocols
70-276 PathHunter RTK Bioassay Kit REV9 User Manual
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