PathHunter® U2OS TrkB-P75 Bioassay Kit

Background Information:

The PathHunter® TrkB-P75 Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses ligand (e.g. BDNF) induced dimerization of TrkB-P75 receptors. This product uses EFC technology. The included cells overexpress ProLink-tagged TrkB and an EA-tagged SH2 domain protein. It also overexpresses an untagged co-receptor, P75. Upon ligand-induced dimerization of TrkB-P75, detectable EFC signal is produced. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates.

Target Class:

Kinase

Family:

RTK

Sub Family:

Protein Tyrosine

Protein Name:

TRKB

Protein Aliases:

GP145-TrkB|Trk-B|Neurotrophic tyrosine kinase receptor type 2|TrkB tyrosine kinase|Tropomyosin-related kinase B

Accession Number:

NM_006180

UniProt Number:

Q16620

Gene Name:

NTRK2

Gene ID:

4915

Gene Aliases:

TRKB

Target Species:

Human

Cell Background:

U2OS