PathHunter® U2OS TrkA-p75 Bioassay Kit

Background Information:

The PathHunter® TrkA-P75 Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses ligand (e.g. b-NGF) induced dimerization of TrkA-P75 receptors. This product uses EFC technology. The included cells overexpress ProLink-tagged TrkA and an EA-tagged SH2 domain protein. It also overexpresses an untagged co-receptor, P75. Upon ligand-induced dimerization of TrkA-P75, detectable EFC signal is produced. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates.

Target Class:

Kinase

Family:

RTK

Sub Family:

Protein Tyrosine

Protein Name:

TRKA

Protein Aliases:

TRK1-transforming tyrosine kinase protein|Tropomyosin-related kinase A|Tyrosine kinase receptor|Tyrosine kinase receptor A|Trk-A|gp140trk|p140-TrkA|high affinity nerve growth factor receptor

Accession Number:

NM_002529

UniProt Number:

P04629

Gene Name:

NTRK1

Gene ID:

4914

Gene Aliases:

TRK|TRKA|MTC

Target Species:

Human

Cell Background:

U2OS