PathHunter® Human TGFBR1/TGFBR2 Dimerization Cell-Based Assay Kit (U2OS)

Background Information:

The PathHunter® Human TGFBR1/TGFBR2 Dimerization Cell-Based Assay Kit (U2OS) utilizes the PathHunter® U2OS TGFBR1/TGFBR2 Dimerization Cell Line. This assay enables the assessment of ligand (e.g. TGF-β1)-induced dimerization of TGFBR1/TGFBR2 pairs. The included cell line employs the PathHunter® Dimerization format using the Enzyme Fragment Complementation (EFC) technology, where the receptor constructs are tagged to enable complementation of the enzyme fragments upon interaction, producing a robust chemiluminescent signal proportional to the dimerization events. The cells overexpress TGFBR1 fused to PK and TGFBR2 fused to EA, enabling ligand-dependent dimerization measurement upon application of the detection kit.
This Kit includes a stable engineered cell line (supplied as two vials of cryopreserved cells); cell line specific reagents for cell thawing, propagation, detachment, cryopreservation, and cell plating; and a 10‑plate detection kit required to run the assay. Assay plates and positive control for use in the assays can be purchased separately. For continued use of the cell line, additional quantities of cell culture and assay reagents are available for purchase separately.
Eurofins DiscoverX optimized Detection Reagents are required for optimal assay performance and must be used with these cell lines in accordance with the cell line license agreed to upon purchase of this cell line assay kit.

Target Class:

Kinase

Family:

TKL

Sub Family:

Protein Ser/Thr

Protein Name:

TGFBR1

Protein Aliases:

activin A receptor type II-like kinase, 53kDa

Accession Number:

NM_004612

UniProt Number:

P36897

Gene Name:

TGFBR1

Gene ID:

7046

Gene Aliases:

ALK-5|ACVRLK4|ALK5|SKR4

Target Species:

Human

Cell Background:

U2OS