The PathHunter® U2OS rhTrkA-rhP75 Functional Assay provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting RTKs. This kit assesses RTK activation via detection of activated rhTrkA and rhP75 dimerization-induced SH2 domain protein recruitment. This product uses EFC technology. The included cell line overexpresses PK-tagged rhTrkA and an EA-tagged SH2 domain protein. It also overexpresses an untagged co-receptor, rhP75 Upon ligand-induced receptor activation, SH2-EA recruitment leads to production of EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. Please note that this product expresses the rhesus version of the protein.
Receptor Tyrosine Kinase
Neurotrophic tyrosine kinase receptor type 1
Stable Cell Lines
Drug Discovery & Development
Store in vapor phase of liquid nitrogen.
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PathHunter® U2OS rhTrkA-rhP75 Functional Assay
The PathHunter® U2OS rhTrkA-rhP75 Functional Assay measures rhTrkA-rhP75 receptor tyrosine kinase activity via ligand-induced SH2 domain protein recruitment. This cell line ships as two vials of cryopreserved cells. Rhesus version.
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