PathHunter® U2OS rhTrkA-rhP75 Functional Assay

The PathHunter® U2OS rhTrkA-rhP75 Functional Assay measures rhTrkA-rhP75 receptor tyrosine kinase activity via ligand-induced SH2 domain protein recruitment. This cell line ships as two vials of cryopreserved cells. Rhesus version.

Catalog #: 93-1009C3 Size: 2 vials

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Biological Information
Background Information:	The PathHunter® U2OS rhTrkA-rhP75 Functional Assay provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting RTKs. This kit assesses RTK activation via detection of activated rhTrkA and rhP75 dimerization-induced SH2 domain protein recruitment. This product uses EFC technology. The included cell line overexpresses PK-tagged rhTrkA and an EA-tagged SH2 domain protein. It also overexpresses an untagged co-receptor, rhP75 Upon ligand-induced receptor activation, SH2-EA recruitment leads to production of EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. Please note that this product expresses the rhesus version of the protein.
Target Class:	Kinase
Family:	Receptor Tyrosine Kinase
Accession Number:	XM_001114243.2
Target Name:	rhTrkA-rhP75
Target Aliases:	Neurotrophic tyrosine kinase receptor type 1
Target Species:	Rhesus Macaque
Cell Background:	U2OS

Usage
Product Type:	Stable Cell Lines
Application:	Drug Discovery & Development
Storage Conditions:	Store in vapor phase of liquid nitrogen.
Usage Disclaimer:	These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.

Assay Information
Assay Type:	Functional
Assay Measures:	Functional Activity
Detection Method:	Chemiluminescence

Additional Information
Brand:	PathHunter®