PathHunter® U2OS rhTrkA-rhP75 Functional Assay

Background Information:

The PathHunter® U2OS rhTrkA-rhP75 Functional Assay provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting RTKs. This kit assesses RTK activation via detection of activated rhTrkA and rhP75 dimerization-induced SH2 domain protein recruitment. This product uses EFC technology. The included cell line overexpresses PK-tagged rhTrkA and an EA-tagged SH2 domain protein. It also overexpresses an untagged co-receptor, rhP75 Upon ligand-induced receptor activation, SH2-EA recruitment leads to production of EFC signal.
This stable engineered cell line is provided as two vials of cryopreserved cells. Please note that this product expresses the rhesus version of the protein.

Target Class:

Kinase

Family:

RTK

Sub Family:

Protein Tyrosine

Protein Name:

TRKA

Protein Aliases:

TRK1-transforming tyrosine kinase protein|Tropomyosin-related kinase A|Tyrosine kinase receptor|Tyrosine kinase receptor A|Trk-A|gp140trk|p140-TrkA|high affinity nerve growth factor receptor

Accession Number:

XM_001114243.2

UniProt Number:

F7DJ75

Gene Name:

NTRK1

Gene ID:

719670

Gene Aliases:

TRK|TRKA|MTC

Target Species:

Rhesus Macaque

Cell Background:

U2OS