Biological Information

Background Information:

The PathHunter® U2OS RANK-IκB Functional Assay is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting the NFκB pathway. This cell line enables detection of IκB levels, allowing for assessment of NFκB pathway activation-induced IκB degradation. This product uses EFC technology. The cell line included overexpresses IκB target protein fused to PL alongside untagged RANK. EA is contained in the detection reagent. Upon pathway-activation-induced degradation of IκB EFC signal is lost.
This stable engineered cell line is provided as two vials of cryopreserved cells.

Target Class:

Signaling Pathway

Family:

Tumour Necrosis Factor (TNF)

Accession Number:

NM_003839.3; NM_020529

Target Species:

Human

Cell Background:

U2OS

Usage

Product Type:

Stable Cell Lines

Application:

Drug Discovery & Development

Storage Conditions:

Store in vapor phase of liquid nitrogen.

Usage disclaimer:

These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.

Assay Information

Assay Type:

Cell Based

Assay Measures:

Functional Activity

Detection Method:

Chemiluminescence

Additional Information

Brand:

PathHunter®