PathHunter® U2OS RANK-IkappaB Functional Assay

The PathHunter® U2OS RANK-IκB Functional Assay measures pathway activation-induced degradation of IκB target protein. This cell line ships as two vials of cryopreserved cells.

Catalog #: 93-0994C3 Size: 2 vials

Biological Information
Background Information:	The PathHunter® U2OS RANK-IκB Functional Assay is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting the NFκB pathway. This cell line enables detection of IκB levels, allowing for assessment of NFκB pathway activation-induced IκB degradation. This product uses EFC technology. The cell line included overexpresses IκB target protein fused to PL alongside untagged RANK. EA is contained in the detection reagent. Upon pathway-activation-induced degradation of IκB EFC signal is lost. This stable modified cell line is provided as two vials of cryopreserved cells.
Target Class:	Signaling Pathway
Family:	Tumor Necrosis Factor Receptor Superfamily
Accession Number:	NM_003839.3
Target Name:	RANK-IκB
Target Aliases:	Tumor necrosis factor receptor superfamilymember 11a
Target Species:	Human
Cell Background:	U2OS

Usage
Product Type:	Stable Cell Lines
Application:	Drug Discovery & Development
Storage Conditions:	Short term (24 h): Store in vapor phase of liquid nitrogen.
Usage Disclaimer:	These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.

Assay Information
Assay Type:	Functional
Assay Measures:	Functional Activity
Detection Method:	Chemiluminescence

Additional Information
Brand:	PathHunter®