PathHunter® U2OS PDGFRb Functional Assay

The PathHunter® U2OS PDGFRb Functional Assay measures PDGFRb receptor tyrosine kinase activity via ligand-induced SH2 domain protein recruitment. This cell line ships as two vials of cryopreserved cells.

Catalog #: 93-0493C3 Size: 2 vials

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Biological Information
Background Information:	The PathHunter® U2OS PDGFRb Functional Assay provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting RTKs. This kit assesses RTK activation via detection of activated PDGFRb dimerization-induced SH2 domain protein recruitment. This product uses EFC technology. The included cell line overexpresses PK-tagged PDGFRb and an EA-tagged SH2 domain protein. Upon ligand-induced receptor activation, SH2-EA recruitment leads to production of EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells.
Target Class:	Kinase
Family:	Receptor Typosine Kinase (RTK): Growth Factor Receptor
Sub Family:	Protein Tyrosine
Accession Number:	NM_002609
Target Name:	PDGFRb
Target Aliases:	Platelet-derived growth factor receptor beta, IMF1, KOGS, IBGC4, JTK12, PDGFR, PENTT, CD140B, PDGFR1, PDGFR-1
Target Species:	Human
Cell Background:	U2OS

Usage
Product Type:	Stable Cell Lines
Application:	Drug Discovery & Development
Storage Conditions:	Store in vapor phase of liquid nitrogen.
Usage Disclaimer:	These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.

Assay Information
Assay Type:	Functional
Assay Measures:	Functional Activity
Detection Method:	Chemiluminescence

Additional Information
Brand:	PathHunter®