PathHunter® U2OS PDGFRb Functional Assay

The PathHunter® U2OS PDGFRb Functional Assay measures PDGFRb receptor tyrosine kinase activity via ligand-induced SH2 domain protein recruitment. This cell line ships as two vials of cryopreserved cells.

Catalog #: 93-0493C3 Size: 2 vials
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Biological Information
Background Information:
The PathHunter® U2OS PDGFRb Functional Assay provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting RTKs. This kit assesses RTK activation via detection of activated PDGFRb dimerization-induced SH2 domain protein recruitment. This product uses EFC technology. The included cell line overexpresses PK-tagged PDGFRb and an EA-tagged SH2 domain protein. Upon ligand-induced receptor activation, SH2-EA recruitment leads to production of EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells.
Target Class:
Kinase
Family:
Receptor Typosine Kinase (RTK): Growth Factor Receptor
Sub Family:
Protein Tyrosine
Accession Number:
NM_002609
Target Name:
PDGFRb
Target Aliases:
Platelet-derived growth factor receptor beta, IMF1, KOGS, IBGC4, JTK12, PDGFR, PENTT, CD140B, PDGFR1, PDGFR-1
Target Species:
Human
Cell Background:
U2OS
Usage
Product Type:
Stable Cell Lines
Application:
Drug Discovery & Development
Storage Conditions:
Store in vapor phase of liquid nitrogen.
Usage Disclaimer:
These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.
Assay Information
Assay Type:
Functional
Assay Measures:
Functional Activity
Detection Method:
Chemiluminescence
Additional Information
Brand:
PathHunter®

User Manuals & Protocols

70-269 PathHunter Receptor Tyrosine Kinase Functional Assay REV4 User Manual

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Datasheets

DRx_DS_93-0493C3_PHCb-RTK_U2OS_PDGFRb_20231013

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