Biological Information
Background Information:
The PathHunter® PDGFRb Bioassay Kit (U2OS) provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This included cells enable assessment of ligand (e.g. PDGF-BB) based activation of PDGFRb receptors via detection of SH2 domain protein recruitment. The included cells overexpress ProLink-tagged PDGFRb target protein and EA-tagged SH2 domain protein. Upon activation of the receptor by its ligand or an agonist antibody, recruitment of SH2 domain protein to the phosphorylated receptor tail leads to production of EFC signal. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates.
Target Class:
Kinase
Family:
RTK
Sub Family:
Protein Tyrosine
Protein Name:
PDGFRbeta
Protein Aliases:
PDGFR-beta|Beta platelet-derived growth factor receptor|Beta-type platelet-derived growth factor receptor|CD140 antigen-like family member B|Platelet-derived growth factor receptor 1|PDGFR-1|CD antigen CD140b
Accession Number:
NM_002609
UniProt Number:
P09619
Gene Name:
PDGFRB
Gene ID:
5159
Gene Aliases:
JTK12|CD140b|PDGFR1|PDGFR
Target Species:
Human
Cell Background:
U2OS
Usage
Product Type:
Bioassay Kits
Application:
Potency, Commercial Release & Stability
Kit Components:
Storage Conditions:
Store in vapor phase of liquid nitrogen.
Usage disclaimer:
These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.
Assay Information
Assay Type:
Cell Based
Assay Measures:
SH2 Recruitment
Detection Method:
Chemiluminescence
Clinical Relevance
Therapeutic Area:
Oncology/Immuno-Oncology
Additional Information
Brand:
PathHunter®