PathHunter® U2OS PDGFRa Bioassay Kit

Background Information:

The PathHunter® U2OS PDGFRa Bioassay Kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. The included cells enable assessment of ligand (e.g. PDGF-BB) based activation of PDGFRa receptors via detection of SH2 domain protein recruitment. The included cell line overexpresses ProLink-tagged PDGFRa target protein and EA-tagged SH2 domain protein. Upon activation of the receptor by its ligand or an agonist antibody, recruitment of SH2 domain protein to the phosphorylated receptor tail leads to production of EFC signal. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates.

Target Class:

Kinase

Family:

RTK

Sub Family:

Protein Tyrosine

Protein Name:

PDGFRalpha

Protein Aliases:

PDGFR-alpha|Alpha platelet-derived growth factor receptor|Alpha-type platelet-derived growth factor receptor|CD140 antigen-like family member A|CD140a antigen|Platelet-derived growth factor alpha receptor|Platelet-derived growth factor receptor 2|PDGFR-2|CD antigen CD140a

Accession Number:

NM_006206.4

UniProt Number:

P16234

Gene Name:

PDGFRA

Gene ID:

5156

Gene Aliases:

CD140a|PDGFR2|GAS9|RHEPDGFRA

Target Species:

Human

Cell Background:

U2OS