| Biological Information | |
|---|---|
| Background Information: | The PathHunter® U2OS PDGFRa Bioassay Kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. The included cells enable assessment of ligand (e.g. PDGF-BB) based activation of PDGFRa receptors via detection of SH2 domain protein recruitment. The included cell line overexpresses ProLink-tagged PDGFRa target protein and EA-tagged SH2 domain protein. Upon activation of the receptor by its ligand or an agonist antibody, recruitment of SH2 domain protein to the phosphorylated receptor tail leads to production of EFC signal. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates. |
| Target Class: | Kinase |
| Family: | Receptor Typosine Kinase (RTK): Growth Factor Receptor |
| Sub Family: | Protein Tyrosine |
| Accession Number: | NM_006206.4 (Alt NC_000004.12) |
| Target Name: | PDGFRa |
| Target Aliases: | PDGFRA, Platelet-derived growth factor receptor alpha, CD140A, PDGFR2, PDGFR-2 |
| Target Species: | Human |
| Cell Background: | U2OS |
| Usage | |
| Product Type: | Bioassay Kits |
| Application: | Potency; Commercial Release & Stability |
| Kit Components: |  |
| Storage Conditions: | Store in vapor phase of liquid nitrogen. |
| Usage Disclaimer: | These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information. |
| Assay Information | |
| Assay Type: | Functional |
| Assay Measures: | SH2 Recruitment |
| Detection Method: | Chemiluminescence |
| Additional Information | |
| Brand: | PathHunter® |
PathHunter® U2OS PDGFRa Bioassay Kit
The PathHunter® PDGFRa Bioassay Kit (U2OS) provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. The included cells enable assessment of ligand (e.g. PDGF-BB) based activation of PDGFRa receptors via detection of SH2 domain protein recruitment.
User Manuals & Protocols
70-276 PathHunter RTK Bioassay Kit REV9 User Manual
View DocumentDatasheets
93-0823Y3x Datasheet
View Document