PathHunter® U2OS p53 Nuclear Translocation Cell Line

The PathHunter® U2OS p53 Nuclear Translocation Cell Line measures p53 DNA damage pathway activation via nuclear translocation of p53. This cell line ships as two vials of cryopreserved cells.

Catalog #: 93-0757C3 Size: 2 vials

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Biological Information
Background Information:	The PathHunter® U2OS p53 Nuclear Translocation Cell Line is a stable, engineered cell line for use in studying drug candidates affecting the p53 DNA damage pathway. This cell line enables assessment of ligand (e.g. camptothecin) based pathway activation via detection of resulting p53 nuclear translocation. This product uses EFC technology. The included cell line overexpresses PL-tagged p53 and nuclear localized EA alongside untagged Keap1. Upon pathway activation, induced nuclear translocation of p53 leads to production of EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells.
Target Class:	Nuclear Protein
Family:	Nuclear Translocation
Accession Number:	NM_000546.2
Target Name:	p53
Target Aliases:	Cellular tumor antigen p53, TP53, tumor protein p53
Target Species:	Human
Cell Background:	U2OS

Usage
Product Type:	Stable Cell Lines
Application:	Drug Discovery & Development
Storage Conditions:	Store in vapor phase of liquid nitrogen.
Usage Disclaimer:	These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.

Assay Information
Assay Type:	Functional
Assay Measures:	Translocation
Detection Method:	Chemiluminescence

Additional Information
Brand:	PathHunter®