PathHunter® U2OS mRHO(P23H) MEM-EA Pharmacotrafficking Assay

The PathHunter® U2OS mRHO(P23H) MEM-EA Pharmacotrafficking Assay measures pharmacochaperone-based stabilization of mutant mRHO GPCR via detection of localization to the membrane. This cell line ships as two vials of cryopreserved cells.

Catalog #: 93-1077C3 Size: 2 vials

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Biological Information
Background Information:	The PathHunter® U2OS mRHO(P23H) MEM-EA Pharmacotrafficking Assay is a stable, engineered cell line for use in studying pharmacochaperones targeting mRHO. This cell line enables assessment of chaperone-based stabilization of mRHO(P23H) via detection of rescued membrane trafficking. This product uses EFC technology. The included cell line overexpresses PK-tagged mutant mRHO(P23H), which is misfolded and retained in the ER. The cell line also expresses membrane-localized EA. When a pharmacochaperone stabilizes the mutant mRHO, it is trafficked to the membrane, producing EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells.
Target Class:	GPCR
Family:	Opsin Receptors
Coupling:	Gt
Accession Number:	NM_145383.1
Target Name:	RHO(P23H)
Target Aliases:	Rhodopsin
Target Species:	Mouse
Cell Background:	U2OS

Usage
Product Type:	Stable Cell Lines
Application:	Pharmacotrafficking
Storage Conditions:	Store in vapor phase of liquid nitrogen.
Usage Disclaimer:	These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.

Assay Information
Assay Type:	Functional
Assay Measures:	Pharmacotrafficking
Detection Method:	Chemiluminescence

Additional Information
Brand:	PathHunter®