PathHunter® U2OS mRHO(P23H) ENDO-EA Pharmacotrafficking Assay

The PathHunter® U2OS mRHO(P23H) ENDO-EA Pharmacotrafficking Assay measures pharmacochaperone-based stabilization of mutant mRHO GPCR via detection of localization to the endosome. This cell line ships as two vials of cryopreserved cells.

Catalog #: 93-0990C3 Size: 2 vials
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Biological Information
Background Information:
The PathHunter® U2OS mRHO(P23H) ENDO-EA Pharmacotrafficking Assay is a stable, engineered cell line for use in studying pharmacochaperones targeting mRHO. This cell line enables assessment of chaperone-based stabilization of mRHO(P23H) via detection of rescued membrane-endosome trafficking. This product uses EFC technology. The included cell line overexpresses PK-tagged mutant mRHO(P23H), which is misfolded and retained in the ER. The cell line also expresses endosome-localized EA. When a pharmacochaperone stabilizes the mutant mRHO, it is trafficked to the membrane and then internalized to the endosome, producing EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells.
Target Class:
GPCR
Family:
Opsin Receptors
Coupling:
Gt
Accession Number:
NM_145383.1
Target Name:
RHO(P23H)
Target Aliases:
Rhodopsin
Target Species:
Mouse
Cell Background:
U2OS
Usage
Product Type:
Stable Cell Lines
Application:
Pharmacotrafficking
Storage Conditions:
Store in vapor phase of liquid nitrogen.
Usage Disclaimer:
These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.
Assay Information
Assay Type:
Functional
Assay Measures:
Pharmacotrafficking
Detection Method:
Chemiluminescence
Additional Information
Brand:
PathHunter®

User Manuals & Protocols

70-302 PathHunter Pharmacotrafficking Assays REV4 User Manual

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Datasheets

93-0990C3 Datasheet

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93-0001
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