PathHunter® U2OS MC4R(T162I) ENDO-EA Pharmacotrafficking Assay

The PathHunter® U2OS MC4R(T162I) ENDO-EA Pharmacotrafficking Assay measures pharmacochaperone-based stabilization of mutant MC4R GPCR via detection of localization to the endosome. This cell line ships as two vials of cryopreserved cells.

Catalog #: 93-1004C3 Size: 2 vials

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Biological Information
Background Information:	The PathHunter® U2OS MC4R(T162I) ENDO-EA Pharmacotrafficking Assay is a stable, engineered cell line for use in studying pharmacochaperones targeting MC4R. This cell line enables assessment of chaperone-based stabilization of MC4R(T162I) via detection of rescued membrane-endosome trafficking. This product uses EFC technology. The included cell line overexpresses PK-tagged mutant MC4R(T162I), which is misfolded and retained in the ER. The cell line also expresses endosome-localized EA. When a pharmacochaperone stabilizes the mutant MC4R, it is trafficked to the membrane and then internalized to the endosome, producing EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells.
Target Class:	GPCR
Family:	Melanocortin Receptors
Sub Family:	Class A
Coupling:	Gs
Accession Number:	NM_005912.2
Target Name:	MC4R
Target Aliases:	Melanocortin receptor 4
Target Species:	Human
Cell Background:	U2OS

Usage
Product Type:	Stable Cell Lines
Application:	Pharmacotrafficking
Storage Conditions:	Store in vapor phase of liquid nitrogen.
Usage Disclaimer:	These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.

Assay Information
Assay Type:	Functional
Assay Measures:	Pharmacotrafficking
Detection Method:	Chemiluminescence

Additional Information
Brand:	PathHunter®