PathHunter® U2OS KISS1R β-Arrestin Cell Line

Background Information:

The PathHunter® KISS1R cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. kisspeptin) based activation of KISS1R GPCR activity via detection of β-arrestin recruitment. This product uses EFC technology. The included cell line overexpresses PK-tagged KISS1R and EA-tagged β-Arrestin 2. Activation of KISS1R stimulates the recruitment of β-Arrestin 2 and produces EFC signal.
This stable engineered cell line is provided as two vials of cryopreserved cells.

Target Class:

GPCR

Family:

Kisspeptin

Sub Family:

Class A

Coupling:

Gq

Protein Name:

KiSS1 (GPR54)

Protein Aliases:

G-protein coupled receptor 54|G-protein coupled receptor OT7T175|hOT7T175|Hypogonadotropin-1|Kisspeptins receptor|Metastin receptor

Accession Number:

NM_032551

UniProt Number:

Q969F8

Gene Name:

KISS1R

Gene ID:

84634

Gene Aliases:

HOT7T175|AXOR12|GPR54

Target Species:

Human

Cell Background:

U2OS