PathHunter® U2OS Keap1-NRF2 Nuclear Translocation Cell Line

The PathHunter® U2OS Keap1-NRF2 Nuclear Translocation Cell Line measures Keap1-NRF2 pathway activation via nuclear translocation of NRF2. This cell line ships as two vials of cryopreserved cells.

Catalog #: 93-0821C3 Size: 2 vials

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Biological Information
Background Information:	The PathHunter® U2OS Keap1-NRF2 Nuclear Translocation Cell Line is a stable, engineered cell line for use in studying drug candidates affecting the Keap1-NRF2 pathway. This cell line enables assessment of ligand (e.g. methyl CDDO) based pathway activation via detection of resulting NRF2 nuclear translocation. This product uses EFC technology. The included cell line overexpresses PL-tagged NRF2 and nuclear localized EA alongside untagged Keap1. Upon pathway activation, induced nuclear translocation of NRF2 leads to production of EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells.
Target Class:	Nuclear Protein
Accession Number:	NM_203500.1
Target Name:	Keap1-NRF2
Target Aliases:	kelch-like ECH-associated protein 1
Target Species:	Human
Cell Background:	U2OS

Usage
Product Type:	Stable Cell Lines
Application:	Drug Discovery & Development
Storage Conditions:	Store in vapor phase of liquid nitrogen.
Usage Disclaimer:	These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.

Assay Information
Assay Type:	Functional
Assay Measures:	Translocation
Detection Method:	Chemiluminescence

Additional Information
Brand:	PathHunter®