PathHunter® U2OS KDR Activity Assay

Background Information:

The PathHunter® KDR RTK Activity cell line is a stable, engineered cell line for use in studying small molecule drug candidates targeting RTKs. This cell line enables assessment of RTK activation via detection of resulting KDR phosphorylation-induced SH2 domain protein recruitment. This product uses EFC technology. The included cell line overexpresses a PK-tagged engineered fusion of the catalytic domain of KDR to the extracellular ligand binding domain of EGFR alongside an EA-tagged SH2 domain protein. Upon ligand induced receptor activation, cross-phosphorylation of the receptor leads to SH2-EA recruitment and production of EFC signal.
This stable engineered cell line is provided as two vials of cryopreserved cells.

Target Class:

Kinase

Family:

RTK

Sub Family:

Protein Tyrosine

Protein Name:

KDR (VEGFR2)

Protein Aliases:

vascular endothelial growth factor receptor 2|fetal liver kinase 1

Accession Number:

NM_005228.3

UniProt Number:

P35968

Gene Name:

KDR

Gene ID:

3791

Gene Aliases:

FLK1|VEGFR|VEGFR2|CD309

Target Species:

Human

Cell Background:

U2OS