PathHunter® U2OS GHR-JAK2 Functional Assay

The PathHunter® U2OS GHR-JAK2 Functional Assay measures GHR cytokine receptor activity via ligand-induced SH2 domain protein recruitment. This cell line ships as two vials of cryopreserved cells.

Catalog #: 93-0829C3 Size: 2 vials
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Biological Information
Background Information:
The PathHunter® GHR-JAK2 Functional assay cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting CTKs. This cell line enables assessment of GHR activation via detection of JAK2 mediated phosphorylation-induced SH2 domain protein recruitment. This product uses EFC technology. The included cell line overexpresses PK-tagged GHR cytokine receptor, an untagged CTK JAK2 and EA-tagged SH2 domain protein. Upon activation of the cytokine receptor, CTK-based receptor phosphorylation leads to SH2-EA recruitment and production of EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells.
Target Class:
Kinase
Family:
Cytosolic Tyrosine Kinase
Accession Number:
NC_000005.10 / NC_000001.11
Target Name:
GHR-JAK2
Target Aliases:
GHBP, GHIP, AIIDEA, JAK1B, JTK3, JAK1
Target Species:
Human
Cell Background:
U2OS
Usage
Product Type:
Stable Cell Lines
Application:
Drug Discovery & Development
Storage Conditions:
Store in vapor phase of liquid nitrogen.
Usage Disclaimer:
These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.
Assay Information
Assay Type:
Functional
Assay Measures:
Functional Activity
Detection Method:
Chemiluminescence
Additional Information
Brand:
PathHunter®

User Manuals & Protocols

70-285 PathHunter Cytosolic Tyrosine Kinase Functional Assay REV4 User Manual

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Datasheets

93-0829C3 Datasheet

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