PathHunter® U2OS FGFR4-β-Klotho Functional Assay

Background Information:

The PathHunter® U2OS FGFR4-β-Klotho Functional Assay provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting RTKs. This kit assesses RTK activation via detection of activated FGFR4 and β-Klotho dimerization-induced SH2 domain protein recruitment. This product uses EFC technology. The included cell line overexpresses PK-tagged FGFR4 and an EA-tagged SH2 domain protein. It also overexpresses an untagged co-receptor, β-Klotho Upon ligand-induced receptor activation, SH2-EA recruitment leads to production of EFC signal.
This stable engineered cell line is provided as two vials of cryopreserved cells.

Target Class:

Kinase

Family:

RTK

Sub Family:

Protein Tyrosine

Protein Name:

FGFR4

Protein Aliases:

CD antigen CD334

Accession Number:

NM_002011

UniProt Number:

P22455

Gene Name:

FGFR4

Gene ID:

2264

Gene Aliases:

JTK2|CD334

Target Species:

Human

Cell Background:

U2OS