PathHunter® U2OS F2RL1 (PAR2, GPR11) β-Arrestin Cell Line

The PathHunter® U2OS F2RL1 (PAR2, GPR11) β-Arrestin Cell Line measures F2RL1 (GPCR) activity via recruitment of β-Arrestin2. This cell line ships as two vials of cryopreserved cells.

Catalog #: 93-0235C3 Size: 2 vials
Inquire
Biological Information
Background Information:
The PathHunter® F2RL1 (PAR2,GPR11) cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. proteases, SLIGRL) based activation of F2RL1 GPCR activity via detection of β-arrestin recruitment. This product uses EFC technology. The included cell line overexpresses PK-tagged F2RL1 and EA-tagged β-Arrestin 2. Activation of F2RL1 stimulates the recruitment of β-Arrestin 2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells.
Target Class:
GPCR
Family:
Proteinase-Activated Receptors
Coupling:
Gi/Go & Gq
Accession Number:
NM_005242.4
Target Name:
F2RL1 (PAR2,GPR11)
Target Aliases:
Coagulation factor II (thrombin) receptor-like 1 (PAR2), F2RL1, PAR2, Proteinase-Activated, GPR11, PAR2
Target Species:
Human
Cell Background:
U2OS
Usage
Product Type:
Stable Cell Lines
Application:
Drug Discovery & Development
Storage Conditions:
Store in vapor phase of liquid nitrogen.
Usage Disclaimer:
These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.
Assay Information
Assay Type:
Functional
β-Arrestin isoform:
β-Arrestin-2
Assay Measures:
β-Arrestin Recruitment
Detection Method:
Chemiluminescence
Additional Information
Brand:
PathHunter®

User Manuals & Protocols

70-247 PathHunter Beta-Arrestin Assay for GPCR Cell Lines REV5 User Manual

View Document

Datasheets

93-0235C3 Datasheet

View Document
PathHunter® Detection Kit
93-0001
Inquire
AssayComplete™ Thawing Reagent T3
92-4103TR
100 mL

$249.00

AssayComplete™ Cell Plating 5 Reagent
93-0563R5A
100 mL

$339.00

AssayComplete™ Freezing Reagent F3
92-5103FR
2 x 50 mL

$339.00

AssayComplete™ Cell Detachment Reagent
92-0009
50 mL

$109.00

AssayComplete™ Cell Culture Kit-103
92-3103G
Kit

$397.00