PathHunter® U2OS CSF3R-JAK1 Functional Assay

The PathHunter® U2OS CSF3R-JAK1 Functional Assay measures CSF3R cytokine receptor activity via ligand-induced SH2 domain protein recruitment. This cell line ships as two vials of cryopreserved cells.

Catalog #: 93-0809C3 Size: 2 vials

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Biological Information
Background Information:	The PathHunter® CSF3R-JAK1 Functional assay cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting CTKs. This cell line enables assessment of CSF3R activation via detection of JAK1 mediated phosphorylation-induced SH2 domain protein recruitment. This product uses EFC technology. The included cell line overexpresses PK-tagged CSF3R cytokine receptor, an untagged CTK JAK1 and EA-tagged SH2 domain protein. Upon activation of the cytokine receptor, CTK-based receptor phosphorylation leads to SH2-EA recruitment and production of EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells.
Target Class:	Kinase
Family:	Cytosolic Tyrosine Kinase
Accession Number:	NM_000760
Target Name:	CSF3R-JAK1
Target Aliases:	colony stimulating factor 3 receptor
Target Species:	Human
Cell Background:	U2OS

Usage
Product Type:	Stable Cell Lines
Application:	Drug Discovery & Development
Storage Conditions:	Store in vapor phase of liquid nitrogen.
Usage Disclaimer:	These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.

Assay Information
Assay Type:	Functional
Assay Measures:	Functional Activity
Detection Method:	Chemiluminescence

Additional Information
Brand:	PathHunter®