PathHunter® U2OS cINSRb Functional Assay

Background Information:

The PathHunter® U2OS cINSRb Functional Assay provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting RTKs. This kit assesses RTK activation via detection of activated cINSRb dimerization-induced SH2 domain protein recruitment. This product uses EFC technology. The included cell line overexpresses PK-tagged cINSRb and an EA-tagged SH2 domain protein. Upon ligand-induced receptor activation, SH2-EA recruitment leads to production of EFC signal.
This stable engineered cell line is provided as two vials of cryopreserved cells. Please note that this product expresses the cyno version of the protein.

Target Class:

Kinase

Family:

RTK

Sub Family:

Protein Tyrosine

Protein Name:

IR

Protein Aliases:

Insulin receptor subunit alpha|INSRa

Accession Number:

XM_005587740.1

UniProt Number:

N/A

Gene Name:

INSR

Gene ID:

102139081

Gene Aliases:

CD220

Target Species:

Cynomolgus Monkey

Cell Background:

U2OS