PathHunter® U2OS CFTR-deltaF508 MEM-EA Pharmacotrafficking Assay

The PathHunter® U2OS CFTR-ΔF508 MEM-EA Pharmacotrafficking Assay measures pharmacochaperone-based stabilization of mutant CFTR GPCR via detection of localization to the membrane. This cell line ships as two vials of cryopreserved cells.

Catalog #: 93-0987C3 Size: 2 vials
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Biological Information
Background Information:
The PathHunter® U2OS CFTR-ΔF508 MEM-EA Pharmacotrafficking Assay is a stable, engineered cell line for use in studying pharmacochaperones targeting CFTR. This cell line enables assessment of chaperone-based stabilization of CFTR-ΔF508 via detection of rescued membrane trafficking. This product uses EFC technology. The included cell line overexpresses PK-tagged mutant CFTR-ΔF508, which is misfolded and retained in the ER. The cell line also expresses membrane-localized EA. When a pharmacochaperone stabilizes the mutant CFTR, it is trafficked to the membrane, producing EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells.
Target Class:
Ion Channel
Family:
Transporter
Sub Family:
Other
Accession Number:
NP_000483.3
Target Name:
CFTR-ΔF508
Target Aliases:
Cystic fibrosis transmembrane conductance regulator
Target Species:
Human
Cell Background:
U2OS
Usage
Product Type:
Stable Cell Lines
Application:
Pharmacotrafficking
Storage Conditions:
Store in vapor phase of liquid nitrogen.
Usage Disclaimer:
These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.
Assay Information
Assay Type:
Functional
Assay Measures:
Pharmacotrafficking
Detection Method:
Chemiluminescence
Additional Information
Brand:
PathHunter®

User Manuals & Protocols

70-302 PathHunter Pharmacotrafficking Assays REV4 User Manual

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Datasheets

93-0987C3 Datasheet

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