PathHunter® U2OS CFTR-deltaF508 MEM-EA Pharmacotrafficking Assay

Background Information:

The PathHunter® U2OS CFTR-ΔF508 MEM-EA Pharmacotrafficking Assay is a stable, engineered cell line for use in studying pharmacochaperones targeting CFTR. This cell line enables assessment of chaperone-based stabilization of CFTR-ΔF508 via detection of rescued membrane trafficking. This product uses EFC technology. The included cell line overexpresses PK-tagged mutant CFTR-ΔF508, which is misfolded and retained in the ER. The cell line also expresses membrane-localized EA. When a pharmacochaperone stabilizes the mutant CFTR, it is trafficked to the membrane, producing EFC signal.
This stable engineered cell line is provided as two vials of cryopreserved cells.

Target Class:

Ion Channel

Family:

Chloride

Sub Family:

Other

Protein Name:

CFTR

Protein Aliases:

ATP-binding cassette sub-family C, member 7

Accession Number:

NP_000483.3

UniProt Number:

P13569

Gene Name:

CFTR

Gene ID:

1080

Gene Aliases:

MRP7|ABC35|TNR-CFTR|dJ760C5.1|CFTR/MRP

Target Species:

Human

Cell Background:

U2OS