PathHunter® U2OS CD86 Ligand Cell Line

Background Information:

The PathHunter® CD86 Ligand cell line is a stable, engineered cell line designed for use with CTLA4 Checkpoint Signaling Cell Lines. When co-cultured, the CD86 expressed in this cell line will induce activity of CTLA4 receptors, which may be assayed through SH2 domain protein recruitment. This product is a companion product designed to work with Immunoglobulin Superfamily Checkpoint Assays. The cell line included overexpresses CD86 cell surface ligand. When co-cultured with the corresponding Checkpoint Signaling Cell Line, the CD86 ligand binds to the checkpoint receptor, inducing receptor activity. This receptor activity leads to recruitment of the relevant SH2 domain protein to the checkpoint receptor inside the receptor cell line and production of EFC signal.
This stable engineered cell line is provided as two vials of cryopreserved cells.

Target Class:

Checkpoint Receptor

Family:

IgSF

Sub Family:

Immune Inhibitory Receptor Ligand

Protein Name:

CD86

Protein Aliases:

B-lymphocyte antigen B7-2

Accession Number:

NM_175862.4

UniProt Number:

P42081

Gene Name:

CD86

Gene ID:

942

Gene Aliases:

B7.2|B7-2

Target Species:

Human

Cell Background:

U2OS