PathHunter® U2OS CD137 Signaling Assay

The PathHunter® U2OS CD137 Signaling Assay measures CD137 signal by activation-induced stabilization of NIK. This cell line ships as two vials of cryopreserved cells.

Catalog #: 93-1089C3 Size: 2 vials

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Biological Information
Background Information:	The PathHunter® CD137 Checkpoint Signaling cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting signaling checkpoints. This cell line enables assessment of CD137 induced non-canonical NF-κB signaling via detection of NIK stabilization. This product uses EFC technology. The included cell line overexpresses ED-tagged NIK (NF-kappa-B Inducing Kinase) protein as well as untagged CD137. Upon activation of the receptor by its ligand or an antibody, NIK is stabilized, allowing detection of EFC signal when treated with exogenous EA. This stable modified cell line is provided as two vials of cryopreserved cells.
Target Class:	Checkpoint Receptor
Family:	TNFR Superfamily Receptor
Sub Family:	CD Molecules
Accession Number:	NM_001561.5
Target Name:	CD137 (4-1BB)
Target Aliases:	4-1BB, TNFRSF9, tumor necrosis factor receptor superfamily member 9
Target Species:	Human
Cell Background:	U2OS

Usage
Product Type:	Stable Cell Lines
Application:	Drug Discovery & Development
Storage Conditions:	Store in vapor phase of liquid nitrogen.
Usage Disclaimer:	These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.

Assay Information
Assay Type:	Functional
Assay Measures:	Signaling
Detection Method:	Chemiluminescence

Additional Information
Brand:	PathHunter®