PathHunter® U2OS c-Ret-GFRα2 Functional Assay

The PathHunter® U2OS c-Ret-GFRα2 Functional Assay measures c-Ret-GFRα2 receptor tyrosine kinase activity via ligand-induced SH2 domain protein recruitment. This cell line ships as two vials of cryopreserved cells. Cyno version.

Catalog #: 93-0816C3 Size: 2 vials
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Biological Information
Background Information:
The PathHunter® U2OS c-Ret-GFRα2 Functional Assay provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting RTKs. This kit assesses RTK activation via detection of activated c-Ret and GFRα2 dimerization-induced SH2 domain protein recruitment. This product uses EFC technology. The included cell line overexpresses PK-tagged c-Ret and an EA-tagged SH2 domain protein. It also overexpresses an untagged co-receptor, GFRα2 Upon ligand-induced receptor activation, SH2-EA recruitment leads to production of EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. Please note that this product expresses the cyno version of the protein.
Target Class:
Kinase
Family:
Receptor Tyrosine Kinase
Accession Number:
NM_020975.4
Target Name:
c-Ret-GFRα2
Target Aliases:
Proto-oncogene tyrosine-protein kinase receptor Ret
Target Species:
Cynomolgus Monkey
Cell Background:
U2OS
Usage
Product Type:
Stable Cell Lines
Application:
Drug Discovery & Development
Storage Conditions:
Store in vapor phase of liquid nitrogen.
Usage Disclaimer:
These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.
Assay Information
Assay Type:
Functional
Assay Measures:
Functional Activity
Detection Method:
Chemiluminescence
Additional Information
Brand:
PathHunter®

User Manuals & Protocols

70-269 PathHunter Receptor Tyrosine Kinase Functional Assay REV4 User Manual

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Datasheets

DRx_DS_93-0816C3_PHCb-RTK_U2OS_c-Ret-GFRalpha2_20231013

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